p2x3 blocking peptides Search Results


p2x3 blocking peptides  (Alomone Labs)
90
Alomone Labs p2x3 blocking peptides
Figure 1 ATP, P2X2, and <t>P2X3</t> in human HNSCC microenvironment and pain. a. Representative HPLC chromatograms showing ATP peaks from tumor and matched normal tissue harvested from the same patient. b. Tumor tissues (Ipsi) had higher levels of ATP compared to matched normal sites (Contra) (n = 10, Student’s t-test). c. Pain scores of functional sharpness and intensity were significantly higher than spontaneous sharpness and intensity, respectively (n = 13, Student’s t-test). d. Mean scores of functional pain (Q2, 4, 6, 7, 8) were significantly higher than spontaneous pain (Q1, 3, 5) (Student’s t-test). e. ATP concentration in extracted cancer tissue correlated positively with mean pain scores (linear regression). f. Representative H&E and immunofluorescence staining (P2X2, P2X3, merged) of a human tongue SCC. Sections were taken from adjacent sections of the SCC. Scale bar: 100 μm.
P2x3 Blocking Peptides, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2x3+blocking+peptides/P2X3+Receptor+Blocking+Peptide/pm24903857-116-20-23
Average 90 stars, based on 1 article reviews
p2x3 blocking peptides - by Bioz Stars, 2026-09
90/100 stars
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p2x3 receptor (extracellular) blocking peptide  (Alomone Labs)
N/A
P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026) is the original antigen used for immunization during Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) generation. The blocking peptide binds and 'blocks' Anti-P2X3 Receptor (extracellular) primary antibody, this makes it a
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Image Search Results


Figure 1 ATP, P2X2, and P2X3 in human HNSCC microenvironment and pain. a. Representative HPLC chromatograms showing ATP peaks from tumor and matched normal tissue harvested from the same patient. b. Tumor tissues (Ipsi) had higher levels of ATP compared to matched normal sites (Contra) (n = 10, Student’s t-test). c. Pain scores of functional sharpness and intensity were significantly higher than spontaneous sharpness and intensity, respectively (n = 13, Student’s t-test). d. Mean scores of functional pain (Q2, 4, 6, 7, 8) were significantly higher than spontaneous pain (Q1, 3, 5) (Student’s t-test). e. ATP concentration in extracted cancer tissue correlated positively with mean pain scores (linear regression). f. Representative H&E and immunofluorescence staining (P2X2, P2X3, merged) of a human tongue SCC. Sections were taken from adjacent sections of the SCC. Scale bar: 100 μm.

Journal: Acta neuropathologica communications

Article Title: Adenosine triphosphate drives head and neck cancer pain through P2X2/3 heterotrimers.

doi: 10.1186/2051-5960-2-62

Figure Lengend Snippet: Figure 1 ATP, P2X2, and P2X3 in human HNSCC microenvironment and pain. a. Representative HPLC chromatograms showing ATP peaks from tumor and matched normal tissue harvested from the same patient. b. Tumor tissues (Ipsi) had higher levels of ATP compared to matched normal sites (Contra) (n = 10, Student’s t-test). c. Pain scores of functional sharpness and intensity were significantly higher than spontaneous sharpness and intensity, respectively (n = 13, Student’s t-test). d. Mean scores of functional pain (Q2, 4, 6, 7, 8) were significantly higher than spontaneous pain (Q1, 3, 5) (Student’s t-test). e. ATP concentration in extracted cancer tissue correlated positively with mean pain scores (linear regression). f. Representative H&E and immunofluorescence staining (P2X2, P2X3, merged) of a human tongue SCC. Sections were taken from adjacent sections of the SCC. Scale bar: 100 μm.

Article Snippet: Control experiments were performed by incubation in secondary antibody alone and by applying P2X2 blocking peptides (Santa Cruz Biotechnology), and P2X3 blocking peptides (Alomone Labs).

Techniques: Functional Assay, Concentration Assay, Immunofluorescence, Staining

Figure 3 HNSCC induces neuronal P2X2/3 plasticity that is reversed by anti-NGF. a. Sustained ATP current (top panel) is enhanced and prolonged following SCC co-culture; anti-NGF added into the co-culture reduced the sustained ATP current. Transient ATP current (lower panel) is not affected by either co-culture or anti-NGF. b. Sustained ATP current density is increased by co-culture (One-way ANOVA), and is reversed by anti-NGF. c. Dot plot of sustained ATP current in different diameter neurons. Co-culture increased current in medium-sized TG neurons; this increased current was reversed by anti-NGF. d. Representative immunofluorescence images of P2X2 and P2X3 expression in TG neurons. e. HNSCC co-culture increased the percentage of neurons expressing P2X2 and P2X3 subunits. Anti-NGF treatment significantly reduced percentage of neurons expressing P2X3 but not P2X2 subunits. The significant increase in the percentage of neurons that express both subunits following co-culture was reversed by anti-NGF application (One-way ANOVA). f. P2X2 immunofluorescence intensity was not changed after co-culture or anti-NGF treatment. P2X3 immunofluorescence intensity was significantly increased following co-culture, and was reduced by anti-NGF (one-way ANOVA). g. In mice with tongue HNSCC, mRNA expression for P2X2 was increased, while P2X3 expression was unchanged in TG neurons (Student’s t-test).

Journal: Acta neuropathologica communications

Article Title: Adenosine triphosphate drives head and neck cancer pain through P2X2/3 heterotrimers.

doi: 10.1186/2051-5960-2-62

Figure Lengend Snippet: Figure 3 HNSCC induces neuronal P2X2/3 plasticity that is reversed by anti-NGF. a. Sustained ATP current (top panel) is enhanced and prolonged following SCC co-culture; anti-NGF added into the co-culture reduced the sustained ATP current. Transient ATP current (lower panel) is not affected by either co-culture or anti-NGF. b. Sustained ATP current density is increased by co-culture (One-way ANOVA), and is reversed by anti-NGF. c. Dot plot of sustained ATP current in different diameter neurons. Co-culture increased current in medium-sized TG neurons; this increased current was reversed by anti-NGF. d. Representative immunofluorescence images of P2X2 and P2X3 expression in TG neurons. e. HNSCC co-culture increased the percentage of neurons expressing P2X2 and P2X3 subunits. Anti-NGF treatment significantly reduced percentage of neurons expressing P2X3 but not P2X2 subunits. The significant increase in the percentage of neurons that express both subunits following co-culture was reversed by anti-NGF application (One-way ANOVA). f. P2X2 immunofluorescence intensity was not changed after co-culture or anti-NGF treatment. P2X3 immunofluorescence intensity was significantly increased following co-culture, and was reduced by anti-NGF (one-way ANOVA). g. In mice with tongue HNSCC, mRNA expression for P2X2 was increased, while P2X3 expression was unchanged in TG neurons (Student’s t-test).

Article Snippet: Control experiments were performed by incubation in secondary antibody alone and by applying P2X2 blocking peptides (Santa Cruz Biotechnology), and P2X3 blocking peptides (Alomone Labs).

Techniques: Co-Culture Assay, Immunofluorescence, Expressing